Study of the chemical composition of essential oils by gas chromatography 309 organic solvent extraction or straight distillation as water steam has a lower cost compared to organic solvents. The chemical compositions of the oil were identified by gc ms and the result as shown in table 1. Chemical composition, physicochemical characteristics, and. The major component of clove oil is usually considered to be eugenol, with. Europe pmc is an archive of life sciences journal literature. The chemical composition on bud, leaf and stem oil of s. Potassium was the predominant mineral, followed by magnesium and calcium.
Gcms analysis of clove syzygium aromaticum bud essential. Origin of clove oil a native of indonesia and the malacca islands, it is an evergreen tree that grows to about 10 meters 30 feet tall and has bright green leaves and nailshaped rosepeach flower buds which turn, upon drying, a. Sixteen volatile compounds were identified from the nhexane extract of the buds of syzygium aromaticum by using gas chromatographymass spectroscopy. In vitro activity of syzygium aromaticum against food. Article pdf available in bioresources 22 may 2007 with 2,564 reads. The antimicrobial activity of spices of human daily diet has been widely demonstrated. The oil yield of whole clove bud and crushed clove bud were 14. Effect of crushing of clove bud syzygium aromaticum and. The relative composition of essential oils was determined and thecompounds were identified by gc. Antioxidant and antibacterial activities of the essential oil. Pdf chemical constituents of clove syzygium aromaticum, f am. The results of these analyses are presented in table i as a relative peak area of each constituent.
Pdf chemical composition and content of essential oil from. Bud, leaf and stem essential oil composition of syzygium. The composition and antifungal activity of clove essential oil eo, obtained from syzygium aromaticum, were studied. Mics, determined according to clinical and laboratory standards institute protocols, and minimum fungicidal concentration. The aim of this study was to analyze the chemical composition of dried clove buds volatile oil in bangladesh by gas chromatographymass spectrometry gc ms technique and also show their medicinal properties according to the characteristics of the constituents present in that essential oil. Nonetheless, the extraction method depends, among other factors, on the kind of material to. Study of the chemical composition of essential oils by gas. Syzygium aromaticum, essential oil, chemical composition, pectinase enzyme 1. Pdf chemical composition and content of essential oil. Syzygium aromaticum, brassica compestris and cow ghee.
Both oils achieved over 85% larval mortality within 24 h. Chemical composition of essential oils the analyses were successful without previous fractionation of essential oils. Syzygium aromaticum showed a lower activity on k562 ic 50 90 ppm, even though this oil did not share the same main compound with cy. Leaf essential oil composition of six syzygium species. The chemical composition of the essential oils of both plants was determined using gc ms while the larvicidal bioassay was carried out using different concentrations of the oils against the larvae of a. Mar 23, 2007 the essential oil extracted from the dried flower buds of clove, eugenia caryophyllata l. Many plant species have been reported to have pharmacological activities attributable to their phytoconstituents such are glycosides, saponins, flavonoids, steroids, tannins, alkaloids, terpenes, etc. Chemical components of the essential oil from the buds and leaves of other species and variant of syzygium had been reported by several workers15 17.
The chemical composition and biological activity of clove essential oil, eugenia caryophyllata syzygium aromaticum l. The chemical composition of clove essential oils was obtained by gas chromatography coupled to a mass spectrometer gc ms, according to the literature, using a qp 2010 shimadzu system equipped with an aoc20i auto injector, rtx5ms silica capillary column 30 m y 0. Chemical composition and antimicrobial activity of the essential oil of syzygium aromaticum flower bud clove against fish systemic bacteria isolated from aquaculture sites. Pharmacologically, clove has been documented as the main source of phenolic molecules like hidroxibenzoic acids, flavonoids, hidroxiphenyl propens, hidroxicinamic acids, and eugenol c 10 h 12 o 2which is the major bioactive moleculeand gallic acid derivatives like hidrolizable tannins that are found in high amounts in the fresh plant table 1 6,9,16. The main constituents of the essential oil are phenylpropanoids such as carvacrol, thymol. Plants have been employed as medicine since time immemorial, and there has been a recent resurgence in the use of plants as medicines due to their little or no toxicity at the doses used for treatment of different ailments. Chemical composition and antioxidant activities of the oleoresin from the clove buds syzygium aromaticum xin li and jianguo xu college of food sciences, shanxi normal university, linfen, china abstract. Chemical composition and antioxidant properties of clove. Chemical composition, antibiotic promotion and in vivo. Secondary metabolites production in clove syzygium. Chemical composition and antimicrobial activity of. Pharmacologically, clove has been documented as the main source of phenolic molecules like hidroxibenzoic acids, flavonoids, hidroxiphenyl propens, hidroxicinamic acids, and eugenol c 10 h 12 o 2which is the major bioactive moleculeand gallic acid derivatives like hidrolizable tannins that are found in high amounts in the fresh plant. The different methods are hereby synthetically presented.
Gc ms separation of compounds from clove essential oil syzygium aromaticum the results showed that the essential oil mainly contained about eugenol 80. Pdf chemical constituents of clove syzygium aromaticum. The essential oil, obtained by hydrodistillation of fruits, was analyzed by gc and gc ms. Isolation of clove syzygium aromaticum compounds thin layer chromatography was used to isolate and separate the clove syzygium aromaticum compounds. Antioxidant and antibacterial activities of the essential. The chemical composition of clove bud oil provided from private turkish company in turkey was investigated. Introduction the clove tree syzygium aromaticum called cengkeh in indonesia, was first cultivated on the island of moluccas, indonesia, in southeast asia 1. Gcms analysis and identifications of chemical constituents. Gcms analysis of clove syzygium aromaticum bud essential oil. Potential of clove of syzygium aromaticum in development of a. The essential oil obtained by hydrodistillation from seeds of syzygium aromaticum myrtaceae growing in benin was analyzed by gas chromatography gc and gas chromatographymass spectrometry gc ms.
Chemical and biological investigations of syzygium aromaticum. Syzygiumaromaticum clove samples using gas chromatography. A total of 26 components were identified accounting for 99. Essential oil composition of syzygium aromaticum l.
This plant is widely used and has commercial value because the content of secondary metabolite compounds. Syzygium aromaticum essential oil is widely used in dental care, as an antiseptic and analgesic and is effective against a large number of bacteria. Chemical composition and antimicrobial activity of the. Chemical composition of the essential oil obtained from the leaves of syzygium caryophyllatum has been analyzed by gc and gc ms twenty four compounds representing 89.
The chemical composition and biological activity of clove. The results as determined by gc ms showed that oil of s. Chemical composition and content of essential oil from the bud of cultivated turkish clove syzygium aromaticum l. Chemical composition and antioxidant activity of essential. Constituents were then identified by comparing the results of the chromatogram and reference retention time using wiley mass spectra library wiley w9n11. Also, it avoids oil heating or the use of sophisticated equipment. Antifungal activity of the clove essential oil from.
Journal of essential oil research 20, 25 4, 315323. The gc ms analysis of clove buds eugenia caryophyllus essential oil led to identification of different compounds fig. Chemical constituents of the essential oil from syzygium. Chemical composition and content of essential oil from the. This paper describes the chemical composition and antimicrobial activity of the essential oil of syzygium aromaticum against vibrio spp. Bud and leaf essential oil composition of syzygium. The chief constituent of clove essential oil is eugenol, which is used as a starting material for the production of vanillin 16.
Larvicidal efficacies and chemical composition of essential. Chemical composition, antioxidant and the possible use as skincare ingredient of clove oil syzygium aromaticum l. In this technology silica gel 2002002 mm plates merk was used. The chemical constituents of clove bud oil were analyzed by using gas chromatographymass spectrometry gc ms. Each has a different chemical composition and flavour. Antifungal activity of the clove essential oil from syzygium. The chemical constituents of clove bud oil were analyzed by using gas chromatographymass spectrometry gcms.
Obtaining the essential oil of syzygium aromaticum. Chemical composition and antimicrobial activity of syzygium. The chemical composition, main physicochemical properties, and nutritional value of seed flour and seed oil of lannea kerstingii were studied. Clove oil syzygium aromaticum information on the origin. Chemical composition and antioxidant properties of clove leaf. This study was designed to perform simultaneous determination of three reference compounds in syzygium aromaticum sa, gallic acid, ellagic acid, and eugenol, and to investigate the chemical antagonistic effect when combining curcuma aromatica ca with sa, based on chromatographic analysis. In this experiment the clove buds essential oil was extracted by hydro. The antibacterial and antioxidant activities were assessed for each oil using minimum inhibitory concentrations mics and 2,2diphenyl1picrylhydrazyl dpph methods. Chemical composition and in vitro cytotoxic screening of.
The oil possesses antiemetic, analgesic, antibacterial, antifungal and anticarcinogenic properties 1015. Clove oil has a warm, strong, spicy smell and the oil is colorless to pale yellow with a medium to watery viscosity. Chemical characterization, antioxidant and antimicrobial. The chemical composition and antioxidant activities of the oleoresin from clove buds were investigated in this work. The chemical composition and biological activity of. To assess the chemical composition and mosquito larvicidal potentials of essential oils of locally sourced pinus sylvestris p. The antibacterial activity of clove syzygium aromaticum essential oil ceo and eugenol was tested against seven gramnegative and nine grampositive fish pathogenic bacteria isolated from cultured olive flounder paralichthys olivaceus in korea. Chemical composition, antibacterial and antioxidant. Differences of particle size did not give significant differences in the yield of clove oil on 24 hour of distillation. The results showed that 22 compositions of the essential oil were identi. The major component of clove essential oils is usually considered to be eugenol, with. Further the clove essential oil was analysed for its chemical components by gc ms. Compounds from syzygium aromaticum possessing growth. This study was conducted to identify qualitatively the chemical composition of essential oil of.
Gcms separation of compounds from clove essential oil syzygium aromaticum the results showed that. The results showed that the essential oils mainly contained about 87. Eugenia caryopyllata is one of the plant that have secondary metabolites that are widely used in the world of health 1. Cloves syzygium aromaticum essential oil is widely used in dental care, as an antiseptic and analgesic and is effective against a large number of bacteria. Pdf chemical composition and content of essential oil from the. Journal of analytical methods in chemistry hindawi. The essential oil of clove syzygium aromaticum obtained by hydrodistillation 0. Clove oil was obtained commercially and analysed by gc and gc ms. Chemical composition identification of essential oil constituents was. A literature survey showed detailed work on chemical composition of bud and leaf essential oils of s. Potential of clove of syzygium aromaticum in development. The chemical composition and antioxidant activities of the essential oil from clove buds toward various oxidative stresses in vitro were investigated in this work. The major components of the essential oil obtained from the buds of syzygium aromaticum were eugenol 71. In this study, clove bud oil, which was cultivated in the mediterranean region of turkey, was provided from a private essential oil company in turkey.
Pcpa01417j19 analysis of the composition of an essential oil, or other volatile liquid, by fast gcfid in french. Yet, their capacity of promoting antibiotic activity has not been explored adequately. The aim of the present study was to investigate the essential oil of. The main secondary metabolites of cloves consist of eugenol. Phytochemical composition and pleotropic pharmacological. Effects of phenological stages on yield and composition of. Chemical composition and antibacterial effects of clove. Chemical composition of the essential oil obtained from the leaves of syzygium caryophyllatum has been analyzed by gc and gcms twenty four compounds representing 89.
Bud, leaf and stem essential oil composition of clove. Clove essential oil rendered the growth of all isolates of c. The eo analysed showed a high content of eugenol 85. Pdf gcms analysis of clove syzygium aromaticum bud. International research journal of pharmacy and medical sciences issn online. Effect of pectinase enzyme on distillation rate of. Chemical and biological investigations of syzygium. In this work, the chemical composition of essential oils of piper nigrum and syzygium aromaticum from chad were determined and their antimicrobial effect in combination with different antibiotics.
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